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For coimmunoprecipitation (co-IP) experiments in HeLa cells, 1 Вg plasmid expressing STAM-GFP, CHMP4B-GFP, or VPS4A-GFP was cotransfected with 1 Вg plasmid expressing full-length FLAG-EphB2-YFP or FLAG-YFP (as negative control) using Lipofectamine 2000 (Thermo Fisher Scientific).



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