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Peptide-specific affinity-purified polyclonal anti-Rabenosyn-5 antibody has been previously described 16rabbit anti-hVps45, mouse anti-Syntaxin6, and mouse anti-Syntaxin16 were purchased from Synaptic Systems (Gottingen, Germany), mouse anti-actin and goat anti-hVps35 antibodies were from Abcam (Cambridge, MA), mouse anti-Rab5 and anti-SNX1 antibodies were from Transduction Laboratories (Lexington, KY), mouse anti-human О1 integrin was from Serotec (Oxford, UK), mouse anti-О-tubulin, mouse anti-GFP was from Roche Applied Science (Indianapolis, IN), mouse anti-HA was from Covance (Berkeley, CA), mouse anti-Myc was from Zymed Laboratories (Carlsbad, CA), and DAPI was from Molecular Probes (Carlsbad, CA). We have reorganized the manuscript and added explanations connection to server has failed windows live the transfected cell experiments shown: (former Figure 7now Figure 4 ), to clarify how these differ from what was shown in the 2008 paper. 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