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HeLa cells in six-well plates were transfected with IST1 expression vectors (2 Оgwell, Lipofectamine 2000; Invitrogen) and siRNA (10 nM, Lipofectamine RNAi MAX; Invitrogen), following the time course t 0, cells seeded at 4 Г- 105 cellswell; t 24 h, IST1 vector; t 32 h, media change IST1 vector; t 44 h, trypsin treatment and reseed reseeded on an upload files to web server php glass coverslip (в3 Г- 104 cells); t 50 h, siRNA; t 56 h, media change siRNA; t 68 h, media change siRNA; and t 98 h, cells fixed and stained with anti-О-tubulin and SYTOX-Green (Invitrogen) and analyzed. В Host them with ease on our high powered and ultra-reliableВ servers!В Our white-labeled Reseller solutions make it easier than ever to launch your own hosting business. 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